Chinese Chemical Letters  2020, Vol. 31 Issue (3): 739-745   PDF    
Synthesis and structure-insecticidal activity relationship of novel phenylpyrazole carboxylic acid derivatives containing fluorine moiety
Baolei Wang*, Hongxue Wang, Hang Liu, Lixia Xiong, Na Yang, Yan Zhang, Zhengming Li*     
State Key Laboratory of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China
Abstract: A series of novel phenylpyrazole carboxylic acid derivatives containing fluorine moiety, i.e., diamides 11, simple aryl-bearing amides 12 and acylthioureas 14 were successfully synthesized based on the key fluoro-containing phenylpyrazole acid intermediate. The new compounds were identified and confirmed by melting point, 1H NMR, 13C NMR and elemental analysis or HRMS. The bioassay results indicated that some of the compounds possessed excellent insecticidal activities towards oriental armyworm, diamondback moth and corn borer at low concentrations. For examples, compounds 11a, 11e-g and 14b exhibited remarkable larvicidal activities with LC50 values of 0.13-0.39 mg/L and 0.0002-0.0014 mg/L against oriental armyworm and diamondback moth, respectively, were comparable with those of the control chlorantraniliprole. Particularly, 11e were found superior to chlorantraniliprole in oriental armyworm tests (LC50:0.23 mg/L vs. 0.26 mg/L); 11a, 11e, 11f and 14c in diamondback moth tests with LC50 values of 0.0002 mg/L, 0.0002 mg/L, 0.0008 mg/L and 0.0005 mg/L, respectively, were more effective than that of chlorantraniliprole. In addition, 12a also showed a promising insecticidal potential and development/optimization advantage. Compounds 11a, 11e-g, 12a, 14b and 14c could be considered as possible new leading structures for further study. The SAR investigation indicated that the compounds with fluorine motif (e.g., -F, -CF2H, -CF3) held apparently favorable insecticidal potentials, which provided useful guidance for further design/development of new phenylpyrazole-containing agrochemicals.
Keywords: Phenylpyrazole carboxylic acid derivatives    Fluorochemicals    Synthesis    Insecticidal activity    Structure-activity relationship    

With the increase of the world population as well as the food, feed and water consumption, and the decrease of the average cultivatable land resources all over the world, how to high efficiently improve the crop production based on the limited farmlands available is becoming an urgent problem. The agrochemicals that are used to control various crop pests, weeds and diseases undoubtedly exert very important roles in solving this increasingly prominent issue. However, there always accompanies negative effects more or less during the use of agrochemicals such as pollution to the eco-environment, long term residue period and the resistance problem. Therefore the innovation for new agrochemicals with novel structures and excellent bioactivities is a permanent subject for the pesticide researchers [1, 2].

N-Pyridylpyrazole derivatives have received continuous concerns since the early of this century owing to their significant biological activities in agriculture applications. The most noticeable agrochemicals of this kind of compounds are the DuPont's famous insecticides chlorantraniliprole and cyantraniliprole (targeting at insect RyR) which both contain the anthranilic diamide and N-pyridylpyrazole structural characteristics [3-5]. Moreover, cyclaniliprole that recently has been developed by Ishihara Sangyo Kaisha is another insecticide of such kind [6, 7]. Because of their potential insecticidal activities towards a variety of Lepidopera, Coleoptera and Diptera pests, and some other good properties as well (high efficiency, ecologically safe, less resistance, etc.), their structural modifications and the bioactivity evaluation of the synthesized compounds have become a research hot topic in recent years [8-16].

Based on the structures of pyridylpyrazole-containing anthranilic diamides, our previous research work have shown that the substitution of Br-containing pyridylpyrazole group with CF3- containing (chloronitro)phenylpyrazole group [17] (Fig. 1, A), or replacing amide bridge by acylthiourea bridge (giving rise to the CF3CH2O-containing pyridylpyrazole acylthiourea derivative) [18] (Fig. 1, B) could retain or increase the insecticidal activities of the parent structure towards the test larvae of Mythimna separata Walker and Plutella xylostella L. at low dose concentrations. Encouraged by these information and with the aim of further looking for new insecticidal agrochemicals, a series of novel phenylpyrazole carboxylic acid derivatives containing fluorine moiety, i.e., diamides 11, simple aryl-bearing amides 12 and acylthioureas 14 were synthesized in this paper based on the key intermediate (chlorofluoro)phenyl(CF3)pyrazole acid (a F effect strategy), which is displayed in Fig. 1. It should be noted that some similar anthranilic diamide analogues have been involved in previous patents by Lahm et al. [19-21], however the new pyrazole carboxylic acid derivatives ((di)amides and acylthioureas) with 2- chloro-4-fluorophenyl motif were first reported in this article. It is also worth noting that several fluorine-containing groups (CH2CF2H, CH2CF3) were introduced into the aliphatic amide part of the structures of these diamide and acylthiourea derivatives (11 and 14), which is an obvious innovation towards the situations in previous literatures [17, 19-22]. The insecticidal activities of all the new synthesized title derivatives were systematically investigated against Mythimna separata Walker, Plutella xylostella L. and O. nubilalis at different dose concentrations in this research. In addition, to further study the structure-activity relationship for the simple aryl-containing amide derivatives 12, chloropyridyl(Br) pyrazole amides 17 as contrasts were also synthesized and bioassayed.

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Fig. 1. The design strategy for the title new phenylpyrazole carboxylic acid derivatives containing fluorine moiety.

As shown the synthetic procedure in Scheme 1, the key intermediate 1-(2-chloro-4-fluorophenyl)-3-(trifluoromethyl)- 1H-pyrazole-5-carboxylic acid (4) was prepared. A mixture of (2-chloro-4-fluorophenyl) hydrazine (1, 140 mmol) and 4, 4, 4- trifluoro-1-(furan-2-yl)butane-1, 3-dione (2, 153 mmol) in glacial acetic acid (150 mL) was refluxed for 5 h with TLC monitoring. After cooling down, the mixture was concentrated under reduced pressure. The residue was added saturated Na2CO3 aq. (50 mL), and then extracted with ethyl acetate (3 × 20 mL). The organic phase was combined, washed with brine and dried over anhydrous Na2SO4, successively. After solvent removal, the residue was conducted to column chromatography with petroleum ether and ethyl acetate (6:1, v/v) as solvents to afford the furylpyrazole intermediate 3 as a yellow sticky oil, yield 84%, 1H NMR (400 MHz, CDCl3): δ 7.51 (dd, 1H, J = 8.8, 5.6 Hz, Ph-H), 7.41 (d, 1H, J =1.2 Hz, furyl-H), 7.32 (dd, 1H, J = 8.0, 2.8 Hz, Ph-H), 7.14–7.19 (m, 1H, Ph-H), 6.93 (s, 1H, pyrazole-H), 6.34 (dd, 1H, J = 3.2, 1.6 Hz, Ph-H), 5.85 (d, 1H, J =3.6 Hz, furyl-H).

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Scheme 1. Synthetic route of the intermediate 4 and the intermediates 7a-f.

To a three-necked flask furylpyrazole intermediate 3 (52.8 mmol), potassium dihydrogen phosphate (68.4 mmol), acetone (100 mL) and water (200 mL) were added. Potassium permanganate (285 mmol) was then added to the above mixture in small portions under stirring. After completion of addition, the reaction systemwas refluxed for 1 h and cooled down. The mixture was filtered. The filtrate was acidized with concentrated hydrochloric acid, then extracted with ethyl acetate (3 ×30 mL). The organic phase was combined and dried over anhydrous Na2SO4. After solvent removal, the residue was conducted to column chromatography using petroleum ether and ethyl acetate (3:1, v/v) as solvents to afford the (chlorofluoro)phenyl(CF3)pyrazole acid intermediate 4 as a white solid, yield 56%, mp 149-150 ℃.1H NMR (400 MHz, CDCl3): δ 8.71 (br, 1H, COOH), 7.45 (dd, 1H, J = 8.8, 5.2 Hz, Ph-H), 7.35 (s, 1H, pyrazole-H), 7.28 (dd, 1H, J = 8.0, 2.4 Hz, Ph-H), 7.12–7.17 (m, 1H, Ph-H).

6, 8-Disubstituted-2H-benzo[d][1, 3]oxazine-2, 4(1H)-dione (6a-d) (Scheme 1) was prepared from 2-amino-3, 5-disubstitutedbenzoic acid (5a-d) and triphosgene following the procedure of Hanusek et al. [23]. 2-Amino-5-chloro-N, 3-dimethylbenzamide (9) was prepared by literature method via SOCl2 acylation and aminolysis successively, based on the starting material 2-amino- 3-methyl-5-chlorobenzoic acid [14]. The intermediate 3-bromo-1- (3-chloropyridin-2-yl)-1H-pyrazole-5-carboxylic acid (15) was prepared via multi steps according to the procedure in literature [18, 24].

2-Amino-3, 5-disubstituted-N-(2, 2-difluoroethyl)/(2, 2, 2-trifluoroethyl)benzamide (7a-f) (Scheme 1) were prepared referring to a similar procedure in literature [14, 23]. A mixture of the benzooxazinedione intermediate 6 (5 mmol), 2, 2-difluoroethan-1- amine or 2, 2, 2-trifluoroethan-1-amine (15 mmol) and 1, 4-dioxane or tetrahydrofuran (30 mL) was reacted for 6 - 8 h with TLC monitoring. After solvent removal under reduced pressure, the residue was purified by recrystallization or column chromatography using petroleum ether/ethyl acetate (5:1, v/v) as solvents to afford the new intermediate 7.

(Chlorofluoro) phenyl(CF3)pyrazole acid 4 (0.8 mmol), oxalyl chloride (3.2 mmol) and dry DCM (15 mL) were mixed and stirred for 2 min at room temperature. Two drops of DMF were added to the above solution. The reaction system was stirred for further 3.5 h at room temperature, and then concentrated under reduced pressure to givecrude intermediate pyrazole carbonyl chloride 8 as a yellow solid, which was directly used to do the next step reaction without purification.

The crude acyl chloride 8 (0.8 mmol) was dissolved in dry DCM (8 mL) and was added dropwise to a stirred solution of 2-amino-5-chloro-N, 3-dimethylbenzamide 9 or 2-amino-3, 5-disubstituted-N-(2, 2-difluoroethyl)/(2, 2, 2-trifluoroethyl)benzamide (7a-f) (0.76 mmol) and Et3N (0.96 mmol) in DCM (20 mL). The system was reacted for 10 h at room temperature, and washed with hydrochloric acid, saturated NaHCO3 aq. and brine, successively. The organic phase was combined and dried over anhydrous Na2SO4. After solvent removal, the residue was conducted to column chromatography using petroleum ether and ethyl acetate (10:1, v/v) as solvents to afford the title compounds 11a-g (Scheme 2) as a white solid.

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Scheme 2. Synthetic route of the title compounds 11a-g, 12a-c and 14a-c.

A solution of 2, 4, 6-trisubstitutedphenylamine (10, 0.8 mmol) in pyridine (10 mL) was added to the flask containing the crude acyl chloride 8 (0.8 mmol) which was prepared from the procedure described above, and the mixture was refluxed for 3 - 5 h. After cooling down, the mixture was poured into water (50 mL) and extracted with ethyl acetate (3×15 mL). The extracts were combined and washed with hydrochloric acid (2×10 mL) and brine, successively. The organic phase was dried over anhydrous Na2SO4. After solvent removal, the residue was further purified by recrystallization from ethanol to afford the title compounds 12a-c (Scheme 2) as a solid.

The crude acyl chloride 8 (0.85 mmol) prepared from the above procedure was dissolved in dry aectonitrile (8 mL) and the solution was added dropwise to a stirred mixture of KSCN (2.13 mmol) and PEG-400 (2 drops) in dry acetonitrile (15 mL). The mixture was stirred for 1 h at room temperature and filtered. To the filtrate (i.e., acyl isothiocyanate 13 in CH3CN), aminobenzamide intermediate 9 or 7d or 7e (0.8 mmol) was added in small portions. The system was reacted for further 4 h at room temperature (TLC monitoring) and concentrated under reduced pressure. The residue was then made purification via column chromatography using petroleum ether and ethyl acetate (4:1, v/v) as solvents to afford the title compounds 14a-c (Scheme 2) as a white solid.

Using the similar synthetic procedure of compounds 12a and b, 3-bromo-N-(2, 4, 6-trisubstitutedphenyl)-1-(3-chloropyridin-2- yl)-1H-pyrazole-5-carboxamide (17a and b) with chloropyridyl (Br)pyrazole acid 15 as acid material (Scheme 3).

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Scheme 3. Synthetic route of the compounds 17a and b.

As shown in Scheme 1, the key intermediate (chlorofluoro) phenyl(CF3)pyrazole acid 4 was first prepared via a nucleophilic addition-elimination-based cyclization of (2-chloro-4-fluorophenyl)hydrazine 1 and trifluoromethyl- and furyl-containing 1, 3-dione 2 in boiling acetic acid and KMnO4-oxidation of the generated new furylpyrazole intermediate 3 with good yield. The nucleophilic substitution reaction of benzooxazinedione intermediate 6 and difluoroethylamine or trifluoroethylamine high effectively gave rise to the new intermediate 7 (76%-95%) (Scheme 1). It is worth noting that the synthesis and the structural characterization of the intermediates 4 and 7 were first reported in this article (Supporting information).

Based on the key acid intermediate 4, the diamide derivatives 11a-g and the amide derivatives 12a-c were smoothly synthesized via (COCl)2-acylation and aminolysis using corresponding aminobenzamide or amine intermediates, respectively(Scheme 2); the acyl chloride intermediate 8 reacted with KSCNunder PEG-400 catalyzing in CH3CN gave the acyl isothiocyanate intermediate 13, which further reacted with aminobenzamide intermediate 9 or 7d or 7e leading to the desired acylthiourea compounds 14a-c (Scheme 2).

Moreover, the amide derivatives 17a and b also could be obtained in satisfactory yield via similar reaction conditions as those of 12a-c, that is, the acyl chloride 16 which derived from the chloropyridyl(Br)pyrazole acid 15 reacted with 2, 4, 6-trisubstitutedphenylamine 10a or 10b in pyridine under reflux for 3 - 5 h (Scheme 3).

The synthesized title compounds were identified and confirmed by melting point, 1H NMR and 13C NMR spectra (Supporting information). The measured elemental analysis or high-resolution mass spectroscopy data were also consistent with the corresponding calculated values (Supporting information). In the 1H NMR of these synthesized new compounds, (di)amide or acylthiourea derivatives, the active proton (NH) signals mainly showed up one or two or three groups of peaks at very downfield with the chemical shift of 6.36–12.12 ppm. As bearing fluoro-containing group, some proton or carbon resonance absorption peaks in most of the compounds obviously appeared characteristic spin coupling and splitting of fluorine atom. For examples, the proton signals of CF2H and CH2 in CH2CF2H-containing compounds 11c and 11e showed up "tt" peaks (2JFH =56.0 Hz, 3JHH =4.0 Hz) at δ~5.90 ppm, and multiplet peaks at 3.36 - 3.75 ppm, respectively; the proton signals of CH2 in CH2CF3-containing compounds 11f and 14c showed up multiplet peaks at 3.60 - 4.40 ppm; the carbon signals of the CF2H and CH2 groups in CH2CF2H-containing compounds 11e, 11 g and 14b appeared at δ~114 ppm and ~42 ppm as triplet peaks (1JFC ≈ 241 Hz) and triplet peaks (2JFC ≈ 26 Hz), respectively; the carbon signals of the CF3 and CH2 groups in CH2CF3-containing compounds 11d, 11f and 14c appeared at δ~123 ppm and ~40 ppm as quartet peaks (1JFC ≈ 279 Hz) and quartet or multiplet peaks (2JFC ≈ 34 Hz), respectively. In addition, in the 13C NMR of acylthiourea derivatives 14a-c, the carbon signals of C=S and two C=O groups were observed at δ~180 ppm, ~166 ppm and ~157 ppm, respectively; around this region, it also appeared doublet peaks at δ~162.5 ppm, ascribing to the benzene carbon that connects to the fluorine atom.

The insecticidal activities of compounds 11, 12, 14 and 17 against oriental armyworm (Mythimna separata Walker), corn borer (O. nubilalis) and diamondback moth (Plutella xylostella L.) were tested in a greenhouse according to the reported methods [17, 18, 25-27], and the detailed procedures are presented in the Supporting information.

As shown in Table 1, initially all the tested compounds exhibited 100% lethality rate against oriental armyworm at a concentration of 100 mg/L. By further determination at lower concentrations, most of the diamide derivatives 11a-g and the acylthiourea derivatives 14a-c could still display remarkable insecticidal activities, e.g., compounds 11a, 11c, 11e-g and 14b possessed 100% larvicidal activities against oriental armyworm at 5 mg/L; compounds 11a, 11e-g and 14b pleasingly showed 20.0%-96.7% activity at 0.25 mg/L. In particular, compounds 11e, 11g and 14b whose lethality rate are 60.0%, 96.7% and 96.7%, respectively, were more effective than the control chlorantraniliprole (50.0%). The (chlorofluoro)phenyl(CF3)pyrazole amide derivatives (with simple phenyl motif) 12a-c and chloropyridyl(Br)pyrazole amide derivatives 17a and b also exhibited favorable insecticidal activities against oriental armyworm. Especially, 12a and 12c at 2.5 mg/L held lethality rate of 33.3% and 30.0%, respectively. From Table 1, it also can be seen that compounds 11a-g, 12a, 12c and 14a-c can totally kill the test larvae of corn borer at 100 mg/L concentration (100%). Remarkably, compounds 11a, 11e-g and 14b whose lethality rates at 5 mg/L are 65.0%, 70.0%, 65.0%, 100% and 100%, respectively were more effective than the control chlorantraniliprole (50.0%).

Table 1
Larvicidal activity of the title compounds and chlorantraniliprole against oriental armyworm (Mythimna separata Walker) and corn borer (O. nubilalis).

As shown in Table 2, all the synthesized compounds displayed excellent insecticidal activities against diamondback moth at 200 mg/L and 100 mg/L concentrations. At lower dosage, compounds 11a, 11e-g, 14b and 14c had 100% insecticidal activities at the concentration of 0.1 mg/L; particularly, these six compounds also possessed 20.0%-75.0% lethality rate at 0.0005 mg/L, near to even higher than that of chlorantraniliprole (40.0%).

Table 2
Larvicidal activity of the title compounds and chlorantraniliprole against diamondback moth (Plutella xylostella L.).

By analyzing the insecticidal activities of the synthesized title compounds corresponding to their structures, the following relationships could be found: (i) When R2 and R3 of compounds 11b-g are fixed as H or Cl and H, respectively, the R1 group in corresponding compounds against oriental armyworm and corn borer shows an activity trend: Cl > CH3 (e.g., in Table 1, 11c > 11b; 11g > 11e). Interestingly, such situation is somewhat different from that against diamondback moth, that is, CH3 > Cl (e.g., in Table 2, 11b > 11c; 11e > 11g). (ii) When R1 = Cl and R2 = H, R3 group in the corresponding compounds against oriental armyworm and corn borer shows the activity sequence of H > F (11c > 11d); when R1 = CH3 and R2 = Cl, the activity trend for R3 group is H > F (11e > 11f; 14b > 14c), that is, CH2CF2H group is better than CH2CF3 group in aliphatic amide motif. (iii) Against diamondback moth, it shows a reverse trend for R3 group, that is F > H (CH2CF3 is better than CH2CF2H). (iv) Comparing the diamide derivatives 11a, 11e and 11f, it was found that CH2CF2H group in the respective types of structures is better than CH3 group for the increase of the insecticidal activities. Such situation is particularly obvious in the cases of 14a and 14b against all the three kinds of test larvae, which indicates the introduction of F atom to the alkyl chain of aliphatic amide motif could greatly increase the insecticidal activity of the parent structures. (v) When bearing the same substituents R1, R2 and R3 in the phenylamine motif, (chlorofluoro)phenyl(CF3) pyrazole group displayed apparent superiority relative to the chloropyridyl(Br)pyrazole group for the contribution to the insecticidal activities (e.g., 12a > 17a; 12b > 17b). Whereas, when R1=Cl, R3=CN both in 12a and b and 17a and b, the R2 group indicates an activity sequence of Cl > NO2.

On the whole, compounds with CH3/Cl, Cl and CONHCH2CF2H groups at benzene ring's 2-, 4- and 6- positions of phenylamine part both in diamide derivatives 11 and acylthiourea derivatives 14 showed the excellent insecticidal activities. In addition, (chlorofluoro)phenyl(CF3)pyrazole amide derivatives 12a and 12c that contain simple groups, i.e., 2, 4-Cl2-6-CN-phenyl and 2, 6-Cl2-4-Fphenyl, respectively, exhibited a favorable and promising insecticidal potentials. Such substituents favorability corresponding to the insecticidal activity or SAR contrast could be well reflected and illustrated as shown in Figs. 2 and 3. An insecticidal activity enhancement of fluorine-containing groups that linked with the aliphatic amide part of both diamide and acylthiourea derivatives was apparently found in Fig. 2 [e.g., 11g (CH2CF3), 11e (CH2CF2H) > 11a (CH3); 14b (CH2CF2H) >> 14a (CH3)]. Also in Fig. 3, when other part of the compounds was fixed as the same, (chlorofluoro)phenyl (CF3)pyrazole has greater contribution than chloropyridyl(Br) pyrazole group for the activity (i.e., 17a > 12a; 17b > 12b). Obviously, the introduction of fluorine moiety both in pyrazole and phenyl rings promoted the insecticidal activity, which is possibly due to the effect of F atom or polyfluoro group by means of improving lipophilicity, metabolic stability and subsequent insecticidal activity of the corresponding compounds [28].

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Fig. 2. The larvicidal activity contrast diagram of compounds 11a, 11e, 11 g, 14a, 14b and chlorantraniliprole (M) against oriental armyworm (Mythimna separata Walker) at different test concentrations.

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Fig. 3. The larvicidal activity contrast diagram of compounds 12a, 12b, 17a and 17b against oriental armyworm (Mythimna separata Walker) at different test concentrations.

Based on the preliminary bioassay results, some compounds with good insecticidal activities were made further investigation on LC50 values. From Table S1 (Supporting information) we can see that compounds 11a, 11e-g and 14b exhibited remarkable larvicidal activities against oriental armyworm with LC50 value of 0.13 - 0.39 mg/L, comparable with that of chlorantraniliprole (0.26 mg/L and 0.11 mg/L at respective batch). Particularly, 11e held LC50 value of 0.23 mg/L (LC95 = 0.88 mg/L), superior to chlorantraniliprole which had a LC50 value of 0.26 mg/L (LC95 = 1.79 mg/L) under the same test conditions. Those compounds mentioned above also exhibited excellent larvicidal activities against diamondback moth with LC50 value of 0.0002 - 0.0014 mg/L (Table S2 in Supporting information). Among which, 11a, 11e, 11f and 14c whose LC50 values are 0.0002 mg/L, 0.0002 mg/L, 0.0008 mg/L and 0.0005 mg/L, respectively, were more effective than that of chlorantraniliprole (0.0014 mg/L and 0.0006 mg/L at respective batch).11g and 14b also had favorable activities with LC50 values of 0.0008 mg/L and 0.0014 mg/L, respectively. In addition, compound 12a possessed LC50 values of 3.41 mg/L and 0.12 mg/L against oriental armyworm and diamondback moth, respectively, showing a promising insecticidal potential and development/optimization advantage considering its comparatively simple structural characteristics.

In summary, a series of novel phenylpyrazole carboxylic acid derivatives containing fluorine moiety, i.e., diamides 11, simple aryl-bearing amides 12 and acylthioureas 14 were successfully synthesized based on the key fluorine-containing phenylpyrazole acid intermediate. The new compounds were identified and confirmed by melting point, 1H NMR, 13C NMR and elemental analysis or HRMS. The bioassay results showed that some of the title compounds possessed excellent insecticidal activities towards oriental armyworm, diamondback moth and corn borer at low concentrations and could be considered as possible new leading insecticides for further study. For examples, compounds 11a, 11e-g and 14b exhibited remarkable larvicidal activities with LC50 value of 0.13 - 0.39 mg/L against oriental armyworm and 0.0002 - 0.0014 mg/L against diamondback moth, respectively, and were comparable with those of the control insecticide chlorantraniliprole. Particularly, 11e held LC50 value of 0.23 mg/L, were superior to chlorantraniliprole in oriental armyworm tests; 11a, 11e, 11f and 14c in diamondback moth tests with LC50 values of 0.0002 mg/L, 0.0002 mg/L, 0.0008 mg/L and 0.0005 mg/L, respectively, were more effective than that of chlorantraniliprole. In addition, simple aryl-bearing amide derivative 12a of the title compounds also showed a promising insecticidal potential and development/optimization advantage. The investigation on structure-insecticidal activity relationship of these novel compounds indicated that the compounds with fluorine motif (e.g., -F, -CF2H, -CF3) held apparently favorable insecticidal potentials, which provided useful guidance for further design/development of new phenylpyrazole-containing agrochemicals.

Acknowledgments

This work was financially supported by the National Key Research and Development Program of China (No. 2017YFD0200505), the National Natural Science Foundation of China (No. 21772103), and Tianjin Natural Science Foundation (No. 17JCYBJC19900). This article is dedicated to the 100th anniversary of Nankai University.

Appendix A. Supplementary data

Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.cclet.2019.07.064.

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