Comparison of the Effect of Chlorine Disinfectant on Staphylococcus Aureus on Disinfection Laboratories of Provincial CDC
SUN Huihui, ZHANG Wei, ZHU Tingting, ZHANG Liubo, BAN Haiqun
Abstract: Objectives To strengthen the quality control of disinfection laboratories and ensure the quality guarantee. Methods Sodium dichloroisocyanurate was selected as the quality control sample. Staphylococcus aureus ATCC 6538 was used as the test strain. According to the requirement of quantitative sterilization test in the Technical standard for disinfection (2002), samples from 32 laboratories of disinfection from provincial, municipal and autonomous regional centers for disease confrol and prevention were randomly divided to conduct a comparison of quality control assessment, robust statistical method to assess the ability of the laboratories. Results 32 laboratories participated in the test and the test results of 22 laboratories were all qualified, which accounted for 68.75%. There were a total of 128 laboratories test data with 104 reached qualification, accounted for 81.25% of them. Conclusions Some laboratories of disinfection showed quality control defects, need to be solved urgently.
Key words: disinfection testing laboratories     comparison     quality control

1 材料与方法 1.1 消毒剂的选择

1.2 中和剂

0.1%硫代硫酸钠+0.1%吐温-80的PBS溶液。

1.3 消毒剂作用剂量及有机干扰物

1.4 试验菌株

1.5 培养基

1.6 试验方法

1.7 统计分析的设计

 $Z = \frac{{x - M}}{{标准IQR}}$

x—检测值，

M—中位数。

1.8 评价标准

|Z|≤2为满意结果；

2＜|Z|＜3为存在问题或可疑的结果；

|Z|≥3为不满意或离群结果。

2 结果 2.1 参加实验室

2.2 消毒剂对金黄色葡萄球菌杀灭实验统计结果

 实验室代码 Z-1 min Z-5 min Z-10 min Z-15 min 实验室代码 Z-1 min Z-5 min Z-10 min Z-15 min 1 -3.52 -6.85 -6.67 -5.81 81 -0.46 0.22 0.45 -0.35 6 3.98 1.47 -0.89 -1.03 86 -0.61 -0.75 -0.82 -0.68 11 5.00 1.23 -0.60 0.46 91 2.84 -2.96 -5.73 -6.10 16 2.36 -3.92 -5.13 -6.05 96 0.00 0.07 -0.24 0.68 21 -0.46 0.41 0.47 -0.17 101 3.98 2.10 -1.36 -1.41 26 -0.21 -1.04 -0.60 -0.90 106 -0.46 0.05 0.53 0.13 31 -0.29 0.25 0.40 0.04 111 0.08 -0.79 0.00 0.35 36 11.74 8.08 4.53 3.06 116 0.21 -0.86 0.75 0.00 41 3.11 -0.94 -1.78 -1.22 121 -0.26 -0.27 0.52 -0.24 46 0.11 -0.53 0.04 -0.57 126 -0.50 0.52 0.59 -0.16 51 0.29 0.00 -2.10 -2.34 131 -0.06 1.31 1.79 0.62 56 1.08 -1.03 -1.01 0.81 141 -0.90 1.57 -0.48 -1.00 61 -1.55 -0.59 1.39 1.11 146 -0.37 0.00 0.33 0.64 66 0.00 -0.48 0.64 0.04 151 -0.40 -0.01 0.30 0.05 71 0.37 2.36 -0.45 0.28 156 0.76 0.33 0.12 0.29 76 -0.38 0.63 -0.38 0.45 161 0 0 0 0

 时间 |Z|＜2 2＜|Z|＜3 |Z|≥3 实验室数 构成比/% 实验室数 构成比/% 实验室数 构成比/% 1 min 2 475.00 2 6.25 6 18.75 5 min 2 681.25 3 9.38 3 9.38 10 min 27 84.38 1 3.13 4 12.5 15 min 27 84.38 1 3.13 4 12.5

3 讨论

 [1] 王戎. 消毒产品卫生监督管理中的问题和对策[J]. 中国卫生产业, 2013(31): 60–61. Wang R. Problems and countermeasures in hygienic supervision and management of disinfection products[J]. China Health Ind, 2013(31): 60–61. (in Chinese). [2] 卫生部卫生法制与监督司. 消毒技术规范[S]. 北京: 中华人民共和国卫生部, 2002. In English:The Ministry of Health Legal System and Supervision. Technical standard for disinfection[S]. Beijing:The Minister of Health of the People's Republic of China, 2002. [3] 中国合格评定国家认可委员会. CNAS-GL02: 2014能力验证结果的统计处理和能力评价指南[S]. 北京: 中国标准出版社, 2014. In English:China National Accreditation Service for Conformity Assessment. CNAS-GL02:2014 Guidance on statistic treatment of proficiency testing results and performance evaluation[S]. Beijing:China Standard Press, 2014.
DOI: 10.13421/j.cnki.hjwsxzz.2017.06.013

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SUN Huihui, ZHANG Wei, ZHU Tingting, ZHANG Liubo, BAN Haiqun

Comparison of the Effect of Chlorine Disinfectant on Staphylococcus Aureus on Disinfection Laboratories of Provincial CDC

Journal of Environmental Hygiene, 2017, 7(6): 469-471
DOI: 10.13421/j.cnki.hjwsxzz.2017.06.013