摘要: 目的 调查河南省开封和安阳市家蝇野外种群对常用化学杀虫剂的抗药性，分析其抗性变化趋势，为家蝇防制提供用药指导。方法 于2012、2014和2016年每年的6－8月在河南省开封和安阳市的垃圾堆、垃圾填埋场、农贸市场等蝇类孳生场所以挥网法采集家蝇成虫，带回实验室饲养后取子一代羽化后3~5 d的雌性家蝇，采用国家标准敏感基线点滴法进行抗性测定。结果 2012、2014和2016年家蝇开封种群对氯菊酯、溴氰菊酯、高效氯氰菊酯的抗性均为高抗，半数致死量（LD₅₀）为0.218 1~2.235 4 μg/只，抗性倍数为62.73~695.00倍；安阳种群对氯菊酯、溴氰菊酯和高效氯氰菊酯的抗性均为高抗，LD₅₀为0.219 4~1.793 8 μg/只，抗性倍数为43.88~765.33倍；2012和2014年2个种群对DDVP均为低抗，2016年开封种群为高抗水平，安阳种群为敏感水平。结论 家蝇开封和安阳种群对拟除虫菊酯类和有机磷类杀虫剂均产生了不同程度的抗性，蝇类防制应坚持以孳生地治理为主的综合防制措施，科学有效地使用化学杀虫剂。

Abstract: Objective Survey the resistance trend of Musca domestica to permethrin, deltamethrin, beta-cypermethrin and DDVP in cities of Kaifeng and Anyang, to provide scientific guidance for chemical control of flies. Methods The adult houseflies were collected in the garbage dumps, landfill and traditional markets of Kaifeng and Anyang from June to August in 2012, 2014 and 2016. The 3 to 5-day-old females from the F₁ generation were used in bioassay. GB recommended sensitive baseline topical application method was used to determine the resistance levels. Results The median lethal dose (LD₅₀) of the Kaifeng population against permethrin, deltamethrin, beta-cypermethrin and DDVP in 2012, 2014, 2016 were 0.218 1-2.235 4 μg/♀, the resistance ratios were 62.73-695.00 fold. The LD₅₀ of the Anyang population against above- mentioned insecticides in 2012, 2014, 2016 were 0.219 4-1.793 8 μg/♀, and the resistance ratios were 43.88-765.33 fold. Low resistance to DDVP was observed in both cities in 2012 and 2014, while high resistance to this pesticide was observed in Kaifeng population in 2016, and high susceptibility was shown in Anyang population in the same year. Resistance trends of Kaifeng and Anyang population were diverse against different insecticides. Conclusion Musca domestica in Kaifeng and Anyang showed different levels of resistance to four commonly used insecticides. The strategy of fly control in Kaifeng and Anyang should focus on source reduction. Chemical control should be limited and implemented with scientific merit.