摘要：目的 通过不同剂量¹³⁷Cs γ射线照射小鼠, 观察不同剂量在不同时间相对机体的损伤情况, 对辐射损伤基础研究的生理指标提供本底数据, 为抗辐射功能性保健食品的研究提供参考指标。方法 以2, 3, 4, 5Gy ¹³⁷Cs γ射线照射KM小鼠, 在照射后3, 5, 7, 14, 21 d分别取样对其血液、造血系统、免疫及遗传系统等生理指标的变化情况进行测试研究, 包括白细胞、微核率、骨髓DNA含量、胸腺指数及脾指数等。结果 研究表明, 白细胞、微核率、骨髓DNA含量、胸腺指数及脾指数等指标与照射强度和时间呈现一定的剂量-时效关系。以照射后3~5 d机体损伤最严重, 而后进入恢复期, 21d基本恢复正常。部分指标与未照射对照组比较差异有显著性。结论 对亚致死剂量γ射线照射下各种生理指标测试的结果是合理的、可靠的; 各种指标用于辐射损伤的研究是可行的, 同样可以用于辐射防护药物的研究。

Abstract: Objective This paper reports the research results of sub-lethal ¹³⁷Cs γ-rays irradiation on Kunming species mice, and the damage degree of different irradiation dosages and stages in mice were determined.The aim is to provide background data for the basic research of physiological indexes about radiation damage, and thus offer reference data for functional health-food of anti-irradiation, as well. Methods The Kunming species mice were irradiated by 2, 3, 4, 5Gy of ¹³⁷Cs γ-rays, and the samples of blood, hematopoietic system, immune system and genetic system were taken and determined, respectively, at 3, 5, 7, 14 and 21 day after irradiation.The varieties of biological indexes at different time were obtained, including the white blood cells (WBC), micro-kernel rate, DNA content of marrow, thymus index and spleen index, and so forth. Results The research manifested that all kinds of the biological indexes showed a relationship between dosage-time response and the irradiation intensity.The damage showed the most severe stage was at 3-5 days after irradiation, and then entered the recovery stage began.It did not get normal until after 21 days.By statistics treatment, some indexes of irradiated mice were of the evident differences compared with the control ones without irradiation. Conclusion Aftere sub-lethal dosages of γ-rays irradiation, the determined results of the various biological indexes in mice were reasonable and reliable.All indexes are available to the research of irradiation damage, and can be applied to the drug research of irradiation protection as well.